CXCL10/IP-10 Recombinant antibody

CXCL10/IP-10 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85307-1-RR
Clone No.242013E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

CXCL10, CXCL10/IP10, IP10, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inIFN gamma, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman ovary cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85307-1-RR targets CXCL10/IP-10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CXCL10/IP-10 fusion protein Eg2489 Predict reactive species
Full Name chemokine (C-X-C motif) ligand 10
Calculated Molecular Weight 11 kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberBC010954
Gene Symbol CXCL10
Gene ID (NCBI) 3627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

Protocols

Product Specific Protocols
WB protocol for CXCL10/IP-10 antibody 85307-1-RRDownload protocol
IHC protocol for CXCL10/IP-10 antibody 85307-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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