CXCL13/BCA1 Polyclonal antibody

CXCL13/BCA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10927-1-AP

Synonyms

ANGIE, ANGIE2, B cell attracting chemokine 1, B lymphocyte chemoattractant, BLC, BLR1L, C X C motif chemokine 13, CXC chemokine BLC, CXCL13, SCYB13, Small inducible cytokine B13



Tested Applications

Positive WB detected inhuman placenta tissue, human plasma tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human renal cell carcinoma tissue, human breast cancer tissue, human lymphoma tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10927-1-AP targets CXCL13/BCA1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL13/BCA1 fusion protein Ag1358
Full Name chemokine (C-X-C motif) ligand 13
Calculated molecular weight 13 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC012589
Gene symbol CXCL13
Gene ID (NCBI) 10563
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLR1 (Burkitt lymphoma receptor-1), also called CXCR5, is highly expressed on Burkitt lymphoma cells and B lymphocytes. It may therefore function in the homing of B lymphocytes to follicles. It was reported that the B cell chemokine CXCL13 is elevated in MS serum and cerebrospinal fluid. CXCL13 is also regarded as a proposed serum biomarker of synovitis in RA( Rheumatoid arthritis). CXCL13 consists of a 22-aa signal peptide and a 87-aa mature poly-peptide.

Protocols

Product Specific Protocols
WB protocol for CXCL13/BCA1 antibody 10927-1-APDownload protocol
IHC protocol for CXCL13/BCA1 antibody 10927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Leuk Res

The role of high serum CXCL13 level in Waldenström macroglobulinemia.

Authors - Yan-Yan Wu
humanIHC

Sci Adv

Defined lifestyle and germline factors predispose Asian populations to gastric cancer.

Authors - Akihiro Suzuki
humanWB

Aging (Albany NY)

Immune profiling reveals prognostic genes in high-grade serous ovarian cancer.

Authors - Yong Wu
humanIHC

J Matern Fetal Neonatal Med

A role for CXCL13 (BCA-1) in pregnancy and intra-amniotic infection/inflammation.

Authors - Nhan-Chang Chia-Ling CL
humanWB

Mol Med Rep

MicroRNA‑186‑5p mediates osteoblastic differentiation and cell viability by targeting CXCL13 in non‑traumatic osteonecrosis.

Authors - Weihua Xu
humanIHC

Biomed Pharmacother

Identification of CXCL13 as a potential biomarker in clear cell renal cell carcinoma via comprehensive bioinformatics analysis.

Authors - Tianbo Xu