CXCR1 Polyclonal antibody

• 

  WB analysis of Jurkat using 55450-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55450-1-AP (CXCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 55450-1-AP

  IP result of anti-CXCR1 (IP:55450-1-AP, 4ug; Detection:55450-1-AP 1:500) with Jurkat cells lysate 4000ug.

• 

  IF Staining of Jurkat using 55450-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR1 antibody (55450-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

CXCR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 55450-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

IL8RA, C C, C C CKR 1, CD128, CD181

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Product Information

55450-1-AP targets CXCR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: interleukin 8 receptor, alpha
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_000634
• Gene Symbol: CXCR1
• Gene ID (NCBI): 3577
• RRID: AB_11182706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25024
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR1, also named CMKAR1, IL8RA, and CD181, belongs to the G-protein coupled receptor 1 family. It is a receptor to interleukin-8, a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. CXCR1 binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. This antibody is specific to CXCR1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Death Dis; CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation; Authors - Wei Zhuang
mouse	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin ; KD Validated
human	WB	Lung Cancer; Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation; Authors - Akio Tada
mouse	WB	Cytokine; IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice.; Authors - Zhihao Wang
mouse	WB	J Agric Food Chem; Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy; Authors - Xiaolei Shi
mouse	WB	Mol Immunol; IL-8 exacerbates CCl4-induced liver fibrosis in human IL-8-expressing mice via the PI3K/Akt/HIF-1α pathway; Authors - Yuchen Ma