CXorf15 Polyclonal antibody

CXorf15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22357-1-AP

Synonyms

Gamma-taxilin, Gamma taxilin, FIAT, Factor inhibiting ATF4-mediated transcription, Environmental lipopolysaccharide-responding gene protein

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Validation Data Gallery

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  WB analysis of mouse kidney using 22357-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22357-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22357-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22357-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22357-1-AP (CXorf15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 22357-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22357-1-AP (CXorf15 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22357-1-AP (CXorf15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22357-1-AP (CXorf15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 22357-1-AP

  Immunofluorescent analysis of HEK-293 cells using 22357-1-AP (CXorf15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse heart tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

22357-1-AP targets CXorf15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXorf15 fusion protein Ag17936
• Full Name: chromosome X open reading frame 15
• Calculated Molecular Weight: 528 aa, 61 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC101576
• Gene Symbol: CXorf15
• Gene ID (NCBI): 55787
• RRID: AB_2879087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma