CFAP47 Polyclonal antibody

CFAP47 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20837-1-AP

Synonyms

CXorf22

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Validation Data Gallery

• 

  WB analysis of mouse lung using 20837-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20837-1-AP (CXorf22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 20837-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue, human lung tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

20837-1-AP targets CFAP47 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CFAP47 fusion protein Ag14910
• Full Name: chromosome X open reading frame 22
• Calculated Molecular Weight: 976 aa, 110 kDa
• GenBank Accession Number: BC027936
• Gene Symbol: CFAP47/CXorf22
• Gene ID (NCBI): 170063
• RRID: AB_11232036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF,CoIP	Mol Hum Reprod; WDR87 interacts with CFAP47 protein in the middle piece of spermatozoa flagella to participate in sperm tail assembly; Authors - Hong-Qing Liao
human	IF	medRxiv; CFAP47 is a novel causative gene implicated in X-linked polycystic kidney disease; Authors - Takayasu Mori