Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24855-1-AP

Synonyms

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

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Validation Data Gallery

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  WB analysis of mouse stomach using 24855-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP (Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 24855-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24855-1-AP (Calponin Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse stomach tissue
• Positive IHC detected in: human heart tissue, human breast hyperplasia tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 24855-1-AP is Calponin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calponin fusion protein Ag21384
• Full Name: calponin 1, basic, smooth muscle
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC022015
• Gene symbol: CNN1
• Gene ID (NCBI): 1264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

Publications

Species	Application	Title
rat	WB	Asian J Androl; The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection.; Authors - Xiao-Feng Yue
mouse	WB	Sci Rep; Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.; Authors - Dongli Liang
human	WB	Mol Ther Nucleic Acids; Long Non-coding RNA PEBP1P2 Suppresses Proliferative VSMCs Phenotypic Switching and Proliferation in Atherosclerosis.; Authors - Xingqiang He