Calponin Polyclonal antibody

Calponin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 24855-1-AP


Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

Tested Applications

Positive WB detected inmouse stomach tissue
Positive IHC detected inhuman heart tissue, human breast hyperplasia tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, C2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 7 publications below

Product Information

The immunogen of 24855-1-AP is Calponin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calponin fusion protein Ag21384
Full Name calponin 1, basic, smooth muscle
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC022015
Gene Symbol CNN1
Gene ID (NCBI) 1264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kDa protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.


Product Specific Protocols
WB protocol for Calponin antibody 24855-1-APDownload protocol
IHC protocol for Calponin antibody 24855-1-APDownload protocol
IF protocol for Calponin antibody 24855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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