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Calponin Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 24855-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Calponin Antibody WB mouse stomach tissue 24855-1-AP

mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24855-1-AP( Calponin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Calponin Antibody IHC human heart tissue 24855-1-AP

Immunohistochemistry of paraffin-embedded human heart tissue slide using 24855-1-AP( Calponin Antibody) at dilution of 1:50 (under 10x lens)

Calponin Antibody IHC human heart tissue 24855-1-AP

Immunohistochemistry of paraffin-embedded human heart tissue slide using 24855-1-AP( Calponin Antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse
Positive WB detected in:
mouse stomach tissue
Positive IHC detected in:
human heart tissue, human breast hyperplasia tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Calponin fusion protein Ag21384
Full name:
calponin 1, basic, smooth muscle
Calculated molecular weight:
33 kDa
Observed molecular weight:
34 kDa
GenBank accession number:
BC022015
Gene ID (NCBI):
1264
Gene symbol
CNN1
Synonyms
Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

Background

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.


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