Carbonic Anhydrase 6/CA6 Polyclonal antibody

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  WB analysis using 32740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32740-1-AP (Carbonic Anhydrase 6/CA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 32740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32740-1-AP (Carbonic Anhydrase 6/CA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Carbonic Anhydrase 6/CA6 Polyclonal Antibody for WB, ELISA

Cat No. 32740-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

CA6, CA VI, Carbonate dehydratase VI, Carbonic anhydrase 6, CA-VI

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Tested Applications

• Positive WB detected in: pig salivary gland tissue, human saliva

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32740-1-AP targets Carbonic Anhydrase 6/CA6 in WB, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Carbonic Anhydrase 6/CA6 fusion protein Eg2963
• Full Name: carbonic anhydrase VI
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_001215.3
• Gene Symbol: CA6
• Gene ID (NCBI): 765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P23280-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA6 (Carbonic anhydrase VI ) is a member of CA family proteins that play a central role in pH regulation and electrolyte balance. CA6 is also known as gustin, is a zinc-containing secreted protein which catalyzes the hydration of carbon hydroxide in saliva. CA6 is specifically expressed in the salivary gland of a number of mammalian species (PMID:28423504). The amino acid sequences are highly conserved across the species. And it was reported that decreasing of CA6 protein was associated with loss of taste and pathological morphology of taste buds (PMID: 9784398).