CPE Polyclonal antibody, PBS Only

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  WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13710-1-AP (same clone as 13710-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CPE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Cat No. 13710-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

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Product Information

13710-1-PBS targets CPE in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4647

  Product name: Recombinant human CPE protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 126-476 aa of BC033866

  Sequence: FLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF

• Full Name: carboxypeptidase E
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC033866
• Gene Symbol: Carboxypeptidase E
• Gene ID (NCBI): 1363
• RRID: AB_2083913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16870
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.