Cleaved Caspase 10/P23 Recombinant antibody

• 

  WB analysis of Jurkat using 85104-3-RR

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85104-3-RR (Cleaved Caspase 10/P23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Cleaved Caspase 10/P23 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85104-3-RR

Clone No.242468G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CASP10, Caspase10, 242468G11, Apoptotic protease Mch 4, Apoptotic protease Mch-4

---

Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85104-3-RR targets Cleaved Caspase 10/P23 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 10, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC042844
• Gene Symbol: Caspase 10
• Gene ID (NCBI): 843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q92851
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). This antibody recognize the cleaved caspase 10.