- Featured Product
- KD/KO Validated
Caspase 7/p20 Polyclonal antibody
Caspase 7/p20 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IP, IHC, IF, ELISA
Cat no : 27155-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells, mouse liver tissue, PC-3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 39 publications below|
|IHC||See 2 publications below|
|IP||See 1 publications below|
The immunogen of 27155-1-AP is Caspase 7/p20 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 7/p20 fusion protein Ag25904|
|Full Name||caspase 7, apoptosis-related cysteine peptidase|
|Calculated molecular weight||303 aa, 34 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC015799|
|Gene ID (NCBI)||840|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
|Product Specific Protocols|
|WB protocol for Caspase 7/p20 antibody 27155-1-AP||Download protocol|
|IF protocol for Caspase 7/p20 antibody 27155-1-AP||Download protocol|
|IP protocol for Caspase 7/p20 antibody 27155-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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