Caspase 7/p20 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 7/p20 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

40

Cat no : 27155-1-AP

Synonyms

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

---

Validation Data Gallery

• 

  WB analysis using 27155-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27155-1-AP

  WB result of Caspase 7 antibody (27155-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 7 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 27155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 27155-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 27155-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27155-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27155-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27155-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 27155-1-AP

  IP result of anti-Caspase 7 (IP:27155-1-AP, 4ug; Detection:27155-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.

• 

  IF Staining of MCF-7 using 27155-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 27155-1-AP (Caspase 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells, mouse liver tissue, PC-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 39 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 27155-1-AP is Caspase 7/p20 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 7/p20 fusion protein Ag25904
• Full Name: caspase 7, apoptosis-related cysteine peptidase
• Calculated molecular weight: 303 aa, 34 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC015799
• Gene symbol: CASP7
• Gene ID (NCBI): 840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Publications

Species	Application	Title
human	WB	J Pineal Res; Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.; Authors - Yue Gao
mouse	WB,IP	Transl Stroke Res; Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule; Authors - Danli Lu
human	WB	Transl Psychiatry; Psychosocial and behavioral problems of children and adolescents in the early stage of reopening schools after the COVID-19 pandemic: a national cross-sectional study in China.; Authors - Lin Wang
rat	WB	Front Cell Dev Biol; Harpagoside Protects Against Doxorubicin-Induced Cardiotoxicity via P53-Parkin-Mediated Mitophagy.; Authors - Weili Li
human	WB,IHC	Drug Deliv; Self-assembled polymeric nanocarrier-mediated co-delivery of metformin and doxorubicin for melanoma therapy.; Authors - Mingming Song
human	WB	Int J Biol Sci; ATIC inhibits autophagy in hepatocellular cancer through the AKT/FOXO3 pathway and serves as a prognostic signature for modeling patient survival.; Authors - Hao Zhang