Cathepsin D antibody (21327-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HCT 116 using 21327-1-AP (same clone as 21327-1-PBS)
WB result of Cathepsin D antibody (21327-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and sg-Cathepsin D transfected HCT-116 cells. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.
WB analysis using 21327-1-AP (same clone as 21327-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.
WB analysis using 21327-1-AP (same clone as 21327-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 21327-1-AP (same clone as 21327-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 21327-1-AP (same clone as 21327-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

21327-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cathepsin D fusion protein Ag15254 Predict reactive species
Full Name	cathepsin D
Calculated Molecular Weight	412 aa, 45 kDa
Observed Molecular Weight	32 kDa, 48 kDa, 52 kDa
GenBank Accession Number	BC016320
Gene Symbol	Cathepsin D
Gene ID (NCBI)	1509
RRID	AB_10733646
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07339
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).