CD2AP Polyclonal antibody

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• CD2AP Polyclonal antibody (24122-1-AP)

CD2AP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24122-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

• 

  WB analysis using 24122-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24122-1-AP (CD2AP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 24122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue, Jurkat cells, K-562 cells, L02 cells
• Positive IHC detected in: mouse kidney tissue, mouse stomach tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24122-1-AP targets CD2AP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21170

  Product name: Recombinant mouse Cd2ap protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-637 aa of BC157901

  Sequence: MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPIKRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKKIRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAPKPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPPPPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKPSLSTPSSASKVNTAAFLTPLELKAKAEADDGKKNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKLKKAVLLS

• Full Name: CD2-associated protein
• Calculated Molecular Weight: 637 aa, 70 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC157901
• Gene Symbol: Cd2ap
• Gene ID (NCBI): 12488
• RRID: AB_2879436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9JLQ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Publications

What published studies show

The CD2AP polyclonal antibody (catalog number 24122-1-AP) has 4 total citations. These appear in the journals Inflammation, American Journal of Physiology—Cell Physiology, Scientific Reports, and Frontiers in Medicine. The associated research fields include podocyte injury and acute kidney injury, podocyte pyroptosis, disulfidptosis in ulcerative colitis and colon adenocarcinoma, and Wnt/β-catenin signaling in podocytes.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Am J Physiol Cell Physiol; Long non-coding RNA Kcnq1ot1 promotes sC5b-9-induced podocyte pyroptosis by inhibiting miR-486a-3p and upregulating NLRP3.; Authors - Chunjian Zhang
mouse	WB	Inflammation; Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.; Authors - Soulixay Senouthai
mouse	WB,IHC	Sci Rep; Disulfidptosis links the pathophysiology of ulcerative colitis and immune infiltration in colon adenocarcinoma; Authors - Chenhao Jiang
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song