Cd2ap Polyclonal antibody

Cd2ap Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24122-1-AP

Synonyms

AL024079, C78928, CD2 associated protein, Cd2ap, METS 1

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Validation Data Gallery

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  WB analysis using 24122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse kidney tissue, K-562 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 24122-1-AP is Cd2ap Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cd2ap fusion protein Ag21170
• Full Name: CD2-associated protein
• Calculated molecular weight: 637 aa, 70 kDa
• Observed molecular weight: 74 kDa
• GenBank accession number: BC157901
• Gene symbol: Cd2ap
• Gene ID (NCBI): 12488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Inflammation; Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.; Authors - Soulixay Senouthai
mouse	WB	Am J Physiol Cell Physiol; Long non-coding RNA Kcnq1ot1 promotes sC5b-9-induced podocyte pyroptosis by inhibiting miR-486a-3p and upregulating NLRP3.; Authors - Chunjian Zhang