Claudin 1 Polyclonal antibody

Claudin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (6)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28674-1-AP

Synonyms

claudin 1, CLD1, CLDN1, ILVASC, SEMP1



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, rat skin tissue, HepG2 cells
Positive IHC detected inhuman skin cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

28674-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat, sheep, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 1 fusion protein Ag29269
Full Name claudin 1
Calculated molecular weight 211 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC012471
Gene symbol CLDN1
Gene ID (NCBI) 9076
RRIDAB_2881190
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Protocols

Product Specific Protocols
WB protocol for Claudin 1 antibody 28674-1-APDownload protocol
IHC protocol for Claudin 1 antibody 28674-1-APDownload protocol
IF protocol for Claudin 1 antibody 28674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Microbiome

Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1

Authors - Hao Chen
mouseWB

J Nanobiotechnology

Human umbilical cord mesenchymal stem cells derived exosome shuttling mir-129-5p attenuates inflammatory bowel disease by inhibiting ferroptosis

Authors - Zhiping Wei
mouseIF

Matrix Biol

Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system

Authors - Gayathri Visakan
human,mouseWB

Biomed Pharmacother

Bacterial butyrate mediates the anti-atherosclerotic effect of silybin

Authors - Hao-Ran Shen
mouseWB

Biomed Pharmacother

Polysaccharide-rich fractions from Enteromorpha prolifera improve hepatic steatosis and gut barrier integrity in high-fat diet-induced obese mice linking to modulation of gut microbiota

Authors - Tiande Zou
mouseIF,WB

Oxid Med Cell Longev

Honokiol Ameliorates DSS-Induced Mouse Colitis by Inhibiting Inflammation and Oxidative Stress and Improving the Intestinal Barrier

Authors - Lu Wang