Claudin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Claudin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

112

Cat no : 28674-1-AP

Synonyms

Caudin 1, CLDN1, SEMP1, ILVASC, CLD1

---

Validation Data Gallery

• 

  WB analysis of A431 using 28674-1-AP

  WB result of Claudin 1 antibody (28674-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 1 transfected A431 cells.

• 

  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 28674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (28674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, rat skin tissue, HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human skin cancer tissue, mouse colon tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Freshly prepared samples are recommended.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 80 publications below
• IHC: See 26 publications below
• IF: See 32 publications below

Product Information

28674-1-AP targets Claudin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 1 fusion protein Ag29269
• Full Name: claudin 1
• Calculated Molecular Weight: 211 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC012471
• Gene Symbol: Claudin 1
• Gene ID (NCBI): 9076
• RRID: AB_2881190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

Publications

Species	Application	Title
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
mouse	WB	J Nanobiotechnology; Human umbilical cord mesenchymal stem cells derived exosome shuttling mir-129-5p attenuates inflammatory bowel disease by inhibiting ferroptosis; Authors - Zhiping Wei
mouse	IF	Matrix Biol; Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system; Authors - Gayathri Visakan
human	WB	Int J Biol Macromol; Anionic polysaccharides benefit the bioavailability of pork myofibrillar protein gels: Evidence from a perspective of protein absorption and metabolism; Authors - Xia Yu
human,mouse	WB	Biomed Pharmacother; Bacterial butyrate mediates the anti-atherosclerotic effect of silybin; Authors - Hao-Ran Shen
mouse	WB	Biomed Pharmacother; Polysaccharide-rich fractions from Enteromorpha prolifera improve hepatic steatosis and gut barrier integrity in high-fat diet-induced obese mice linking to modulation of gut microbiota; Authors - Tiande Zou