Claudin 1 Polyclonal antibody

Claudin 1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 28674-1-AP

Synonyms

claudin 1, CLD1, CLDN1, ILVASC, SEMP1

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Validation Data Gallery

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 28674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 28674-1-AP (Claudin 1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, rat skin tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

28674-1-AP targets Claudin 1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 1 fusion protein Ag29269
• Full Name: claudin 1
• Calculated molecular weight: 211 aa, 23 kDa
• Observed molecular weight: 20-23 kDa
• GenBank accession number: BC012471
• Gene symbol: CLDN1
• Gene ID (NCBI): 9076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.