Claudin 1 Polyclonal antibody
Claudin 1 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28674-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse skin tissue, rat skin tissue |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
28674-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Claudin 1 fusion protein Ag29269 |
Full Name | claudin 1 |
Calculated molecular weight | 211 aa, 23 kDa |
Observed molecular weight | 20-23 kDa |
GenBank accession number | BC012471 |
Gene symbol | CLDN1 |
Gene ID (NCBI) | 9076 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.
Protocols
Product Specific Protocols | |
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WB protocol for Claudin 1 antibody 28674-1-AP | Download protocol |
IHC protocol for Claudin 1 antibody 28674-1-AP | Download protocol |
IF protocol for Claudin 1 antibody 28674-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Bioengineered Oxycodone protects cardiac microvascular endothelial cells against ischemia/reperfusion injury by binding to Sigma-1 Receptor. | ||
Front Physiol Gut Microbiota Was Involved in the Process of Liver Injury During Intra-Abdominal Hypertension. | ||
Int Immunopharmacol Gastrodia remodels intestinal microflora to suppress inflammation in mice with early atherosclerosis. | ||
Front Immunol Inhibitor of Differentiation-2 Protein Ameliorates DSS-Induced Ulcerative Colitis by Inhibiting NF-κB Activation in Neutrophils. | ||
Matrix Biol Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system |