Connexin 43 Polyclonal antibody

Connexin 43 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 26980-1-AP


Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, human heart tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26980-1-AP is Connexin 43 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin 43 fusion protein Ag25533
Full Name gap junction protein, alpha 1, 43kDa
Calculated molecular weight 43 kDa
Observed molecular weight 43-47 kDa
GenBank accession numberBC026329
Gene symbol GJA1
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).


Product Specific Protocols
WB protocol for Connexin 43 antibody 26980-1-APDownload protocol
IHC protocol for Connexin 43 antibody 26980-1-APDownload protocol
IF protocol for Connexin 43 antibody 26980-1-APDownload protocol
IP protocol for Connexin 43 antibody 26980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Mol Biol Lett

Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions

Authors - Seyram Yao Adzraku

Free Radic Biol Med

SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics

Authors - Rui Wang

J Virol

Human Cytomegalovirus IE1 Impairs Neuronal Migration by Downregulating Connexin 43

Authors - Sheng-Nan Huang

Int J Mol Sci

Different Contacted Cell Types Contribute to Acquiring Different Properties in Brain Microglial Cells upon Intercellular Interaction

Authors - Akiko Nakano-Doi

Stem Cells

Gap junction-mediated cell-cell interaction between transplanted mesenchymal stem cells and vascular endothelium in stroke.

Authors - Akie Kikuchi-Taura

Stem Cell Res Ther

circAKT3 positively regulates osteogenic differentiation of human dental pulp stromal cells via miR-206/CX43 axis.

Authors - Bo Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Muhammad (Verified Customer) (08-30-2022)

Cx43 antibody used is excellent and working in HUVEC cells.

  • Applications: Western Blot
  • Cell Tissue Type: HUVEC

ABDULLAH (Verified Customer) (11-08-2019)

This antibody worked great!It detected the protein at the right size.There wasn't any background.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: BEND.3 cells