Connexin 43 Polyclonal antibody
Connexin 43 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, IF
Conjugate
Unconjugated
Cat no : 26980-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, mouse testis tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | mouse heart tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse heart tissue, human heart tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 13 publications below |
IHC | See 4 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
Product Information
The immunogen of 26980-1-AP is Connexin 43 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, pig, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Connexin 43 fusion protein Ag25533 |
Full Name | gap junction protein, alpha 1, 43kDa |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 43-47 kDa |
GenBank accession number | BC026329 |
Gene symbol | GJA1 |
Gene ID (NCBI) | 2697 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).
Protocols
Product Specific Protocols | |
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WB protocol for Connexin 43 antibody 26980-1-AP | Download protocol |
IHC protocol for Connexin 43 antibody 26980-1-AP | Download protocol |
IF protocol for Connexin 43 antibody 26980-1-AP | Download protocol |
IP protocol for Connexin 43 antibody 26980-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Ther Med miR-206 regulates alveolar type II epithelial cell Cx43 expression in sepsis-induced acute lung injury. | ||
Genes Cells Lipoprotein receptor-related protein 6 is required to maintain intercalated disc integrity. | ||
Andrologia Inhibition of gap junction communication between cells can induce apoptosis of corpus cavernosum smooth muscle in guinea pigs. | ||
Endocr J Contraction-associated proteins expression by human uterine smooth muscle cells depends on maternal serum and progranulin associated with gestational weight gain. | ||
Cardiovasc Ther LCZ696 Therapy Reduces Ventricular Tachyarrhythmia Inducibility in a Myocardial Infarction-Induced Heart Failure Rat Model. | ||
Pulm Pharmacol Ther The promoting role of Cx43 on the proliferation and migration of arterial smooth muscle cells for angiotensin II-dependent hypertension. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH ABDULLAH (Verified Customer) (11-08-2019) | This antibody worked great!It detected the protein at the right size.There wasn't any background.
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