Connexin 43 Polyclonal antibody

Connexin 43 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26980-1-AP

Synonyms

Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD



Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, human heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26980-1-AP is Connexin 43 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin 43 fusion protein Ag25533
Full Name gap junction protein, alpha 1, 43kDa
Calculated molecular weight 43 kDa
Observed molecular weight 43-47 kDa
GenBank accession numberBC026329
Gene symbol GJA1
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Protocols

Product Specific Protocols
WB protocol for Connexin 43 antibody 26980-1-APDownload protocol
IHC protocol for Connexin 43 antibody 26980-1-APDownload protocol
IF protocol for Connexin 43 antibody 26980-1-APDownload protocol
IP protocol for Connexin 43 antibody 26980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,CoIP

Invest Ophthalmol Vis Sci

High Glucose Aggravates Retinal Endothelial Cell Dysfunction by Activating the RhoA/ROCK1/pMLC/Connexin43 Signaling Pathway

Authors - Hongran Zhao
ratWB,IF

Front Mol Neurosci

NF-κB and AP-1 are required for the lipopolysaccharide-induced expression of MCP-1, CXCL1, and Cx43 in cultured rat dorsal spinal cord astrocytes

Authors - Ying Lu
mouse,humanIHC,IF

Reprod Biol Endocrinol

Metabonomics profile analysis in inflammation-induced preterm birth and the potential role of metabolites in regulating premature cervical ripening

Authors - Yan Yan
ratWB,IF

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
mouseWB

Exp Ther Med

miR-206 regulates alveolar type II epithelial cell Cx43 expression in sepsis-induced acute lung injury.

Authors - Jiawei Zhou
mouseWB,IF,IP

Genes Cells

Lipoprotein receptor-related protein 6 is required to maintain intercalated disc integrity.

Authors - Xiang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Muhammad (Verified Customer) (08-30-2022)

Cx43 antibody used is excellent and working in HUVEC cells.

  • Applications: Western Blot
  • Cell Tissue Type: HUVEC
FH

ABDULLAH (Verified Customer) (11-08-2019)

This antibody worked great!It detected the protein at the right size.There wasn't any background.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: BEND.3 cells