|Positive WB detected in||mouse brain tissue, mouse testis tissue, mouse heart tissue, rat brain tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||mouse heart tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue, human heart tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26980-1-AP is Connexin 43 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Connexin 43 fusion protein Ag25533|
|Full Name||gap junction protein, alpha 1, 43kDa|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43-47 kDa|
|GenBank accession number||BC026329|
|Gene ID (NCBI)||2697|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).
|Product Specific Protocols|
|WB protocol for Connexin 43 antibody 26980-1-AP||Download protocol|
|IHC protocol for Connexin 43 antibody 26980-1-AP||Download protocol|
|IF protocol for Connexin 43 antibody 26980-1-AP||Download protocol|
|IP protocol for Connexin 43 antibody 26980-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exp Ther Med
miR-206 regulates alveolar type II epithelial cell Cx43 expression in sepsis-induced acute lung injury.
Lipoprotein receptor-related protein 6 is required to maintain intercalated disc integrity.
Contraction-associated proteins expression by human uterine smooth muscle cells depends on maternal serum and progranulin associated with gestational weight gain.
LCZ696 Therapy Reduces Ventricular Tachyarrhythmia Inducibility in a Myocardial Infarction-Induced Heart Failure Rat Model.
Protective effects of betaine on diabetic induced disruption of the male mice blood-testis barrier by regulating oxidative stress-mediated p38 MAPK pathways.
Contribution of Connexin Hemichannels to the Pathogenesis of Acute Lung Injury.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
ABDULLAH (Verified Customer) (11-08-2019)
This antibody worked great!It detected the protein at the right size.There wasn't any background.