Connexin 45 Polyclonal antibody, PBS Only

• 

  WB analysis using 22184-1-AP (same clone as 22184-1-PBS)

  mouse heart tissue was subjected to SDS PAGE followed by western blot with 22184-1-AP (Connexin 45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 22184-1-AP (same clone as 22184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22184-1-AP (Connexin 45 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22184-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Connexin 45 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 22184-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7

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Product Information

22184-1-PBS targets Connexin 45 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16742

  Product name: Recombinant human GJC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 248-396 aa of BC096213

  Sequence: WEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKNSVWI

• Full Name: gap junction protein, gamma 1, 45kDa
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC096213
• Gene Symbol: Connexin-45
• Gene ID (NCBI): 10052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36383
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Connexin 45 (Cx45), also known as Gap junction gamma protein (GJC1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. A loss-of-function mutation in Cx45 contributing to Atrial fibrillation (AF) and other cardiac arrhythmias (PMID: 33429106).