Connexin 45 Polyclonal antibody

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  WB analysis using 22184-1-AP

  mouse heart tissue was subjected to SDS PAGE followed by western blot with 22184-1-AP (Connexin 45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 22184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22184-1-AP (Connexin 45 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Connexin 45 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22184-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

22184-1-AP targets Connexin 45 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16742

  Product name: Recombinant human GJC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 248-396 aa of BC096213

  Sequence: WEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKNSVWI

• Full Name: gap junction protein, gamma 1, 45kDa
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC096213
• Gene Symbol: Connexin-45
• Gene ID (NCBI): 10052
• RRID: AB_3085686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36383
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin 45 (Cx45), also known as Gap junction gamma protein (GJC1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. A loss-of-function mutation in Cx45 contributing to Atrial fibrillation (AF) and other cardiac arrhythmias (PMID: 33429106).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Appl Biochem Biotechnol; Cardiac Fibroblasts Enhance MMP2 Activity to Suppress Gap Junction Function in Cardiomyocytes; Authors - Xue Bai