Cxcl15 Polyclonal antibody

Cxcl15 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31087-1-AP

Synonyms

Cxcl15, lungkine, Scyb15, weche

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Validation Data Gallery

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  IP experiment of mouse lung using 31087-1-AP

  IP result of anti-Cxcl15 (IP:31087-1-AP, 4ug; Detection:31087-1-AP 1:500) with mouse lung tissue lysate 1120 ug.

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  IHC staining of mouse lung using 31087-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 31087-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31087-1-AP targets Cxcl15 in IP, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cxcl15 fusion protein Ag33964
• Full Name: chemokine (C-X-C motif) ligand 15
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC061138
• Gene Symbol: Cxcl15
• Gene ID (NCBI): 20309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cxcl15, also known as lungkine or WECHE, is a small cytokine belonging to the CXC chemokine family that has been described in the mouse. High levels of Cxcl15 mRNA were specifically detected in the lung and at lower levels in fetal lung tissue by Northern blot and in situ hybridization, suggesting a potential role for this chemokine during lung development. Moreover, the Cxcl15 protein is secreted into the airway spaces and induces the in vitro and in vivo migration of neutrophils, suggesting that it is involved in lung-specific neutrophil trafficking.