Cytochrome P450 Reductase Polyclonal antibody, PBS Only

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• Cytochrome P450 Reductase Polyclonal antibody, PBS Only (29814-1-PBS)

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  WB analysis using 29814-1-AP (same clone as 29814-1-PBS)

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 29814-1-AP (same clone as 29814-1-PBS)

  WB result of Cytochrome P450 Reductase antibody (29814-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome P450 Reductase transfected HepG2 cells. This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29814-1-AP (same clone as 29814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 29814-1-AP (same clone as 29814-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 29814-1-AP (same clone as 29814-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Cytochrome P450 Reductase Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29814-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

POR, P450R, CYPOR, CPR

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Product Information

29814-1-PBS targets Cytochrome P450 Reductase in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30899

  Product name: Recombinant human POR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 43-216 aa of NM_000941

  Sequence: LFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF

• Full Name: P450 (cytochrome) oxidoreductase
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: NM_000941
• Gene Symbol: Cytochrome P450 Reductase
• Gene ID (NCBI): 5447
• RRID: AB_3086171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16435
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa.