CoraLite® Plus 488-conjugated Cytokeratin 13 Monoclonal antibody

Cytokeratin 13 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10G2

Cat no : CL488-66684

Synonyms

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13

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Validation Data Gallery

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  IF Staining of human cervical cancer using CL488-66684

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite® Plus 488 Cytokeratin 13 antibody (CL488-66684, Clone: 1E10G2 ) at dilution of 1:100.

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  IF Staining of human cervical cancer using CL488-66684

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite® Plus 488 Cytokeratin 13 antibody (CL488-66684, Clone: 1E10G2 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: human cervical cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66684 targets Cytokeratin 13 in IF applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Full Name: keratin 13
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002661
• Gene Symbol: KRT13
• Gene ID (NCBI): 3860
• RRID: AB_2919357
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia.