Cytokeratin 13 Monoclonal antibody

Cytokeratin 13 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1E10G2

Cat no : 66684-1-Ig

Synonyms

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13



Tested Applications

Positive WB detected inA431 cells, rat skin tissue
Positive IHC detected inhuman cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66684-1-Ig targets Cytokeratin 13 in WB, IHC, FC,ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217
Full Name keratin 13
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002661
Gene symbol KRT13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
IHC protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
FC protocol for Cytokeratin 13 antibody 66684-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols