CoraLite® Plus 488-conjugated Cytokeratin 20 Recombinant antibody

Cytokeratin 20 Recombinant Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H10

Cat no : CL488-82428

Synonyms

CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

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Validation Data Gallery

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  IF Staining of HT-29 using CL488-82428

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-82428, Clone: 2H10 ) at dilution of 1:200.

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  FC experiment of HT-29 using CL488-82428

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cytokeratin 20 (CL488-82428, Clone:2H10) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HT-29 cells
• Positive FC detected in: HT-29 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82428 targets Cytokeratin 20 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 20 fusion protein Ag11308
• Full Name: keratin 20
• Calculated Molecular Weight: 424 aa, 48 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC031559
• Gene Symbol: KRT20
• Gene ID (NCBI): 54474
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.