NSG1 Polyclonal antibody

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  WB analysis using 19923-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19923-1-AP (NSG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NSG1 Polyclonal Antibody for WB, ELISA

Cat No. 19923-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

D4S234E, D4S234, NEEP21, Neuron-enriched endosomal protein of 21 kDa, Neuron-specific protein family member 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19923-1-AP targets NSG1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13888

  Product name: Recombinant human D4S234E protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-185 aa of BC001745

  Sequence: MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA

• Full Name: DNA segment on chromosome 4 (unique) 234 expressed sequence
• Calculated Molecular Weight: 185 aa, 21 kDa
• Observed Molecular Weight: 16-21 kDa
• GenBank Accession Number: BC001745
• Gene Symbol: D4S234E
• Gene ID (NCBI): 27065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42857
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSG1, also known as Neuron-Enriched Endosomal Protein of 21 kDa (NEEP21) , is a protein encoded by the NSG1 gene located on human chromosome 4p16.3 . The Neuron-Specific Gene (NSG) family includes NSG1, NSG2, and NSG3. As their name indicates they are expressed almost exclusively in neurons, and localize to multiple subcellular organelles including the Golgi apparatus, early and late endosomes, and the plasma membrane. As a highly enriched endosomal protein within neuronal cells, NSG1 has been discovered to facilitate the process of epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC).(PMID: 37872157,PMID: 41415429)