Dystroglycan Polyclonal antibody

Dystroglycan Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11017-1-AP

Synonyms

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11017-1-AP targets Dystroglycan in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dystroglycan fusion protein Ag1456
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Calculated molecular weight 97 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC012740
Gene symbol DAG1
Gene ID (NCBI) 1605
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

Protocols

Product Specific Protocols
WB protocol for Dystroglycan antibody 11017-1-APDownload protocol
IHC protocol for Dystroglycan antibody 11017-1-APDownload protocol
IF protocol for Dystroglycan antibody 11017-1-APDownload protocol
IP protocol for Dystroglycan antibody 11017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Med Sci Monit

Matrix Metalloproteases-Mediated Cleavage on β-Dystroglycan May Play a Key Role in the Blood-Brain Barrier After Intracerebral Hemorrhage in Rats.

Authors - Xin Zhang
mouseIF

Biochem Biophys Rep

PMID: 29124203

Authors - Yuko Matsuura-Hachiya
humanWB

Cell Commun Signal

Wnt-driven LARGE2 mediates laminin-adhesive O-glycosylation in human colonic epithelial cells and colorectal cancer.

Authors - Vanessa Dietinger
mouseIHC

Nat Commun

IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models.

Authors - Shao-Wei Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (01-10-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina
Dystroglycan Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:11017-1-AP)