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Dystroglycan Polyclonal antibody

Dystroglycan Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11017-1-AP


156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Alpha-Dystroglycan, Beta DG, Beta dystroglycan, Beta-dystroglycan, DAG, DAG1, Dystroglycan

Tested Applications

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, mouse brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11017-1-AP targets Dystroglycan in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dystroglycan fusion protein Ag1456
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC012740
Gene Symbol DAG1
Gene ID (NCBI) 1605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).


Product Specific Protocols
WB protocol for Dystroglycan antibody 11017-1-APDownload protocol
IHC protocol for Dystroglycan antibody 11017-1-APDownload protocol
IF protocol for Dystroglycan antibody 11017-1-APDownload protocol
IP protocol for Dystroglycan antibody 11017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Front Cardiovasc Med

Determination of Agrin and Related Proteins Levels as a Function of Age in Human Hearts.

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J Med Virol

Identification of potential plasma markers for hepatitis B virus-related chronic hepatitis and liver fibrosis/cirrhosis.

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Perrin (Verified Customer) (11-22-2022)

Works very well, with little background

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 to 100
  • Cell Tissue Type: Gut slice, whole mount

Ryan (Verified Customer) (01-10-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina
Dystroglycan Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:11017-1-AP)