|Positive WB detected in||mouse brain tissue, HeLa cells, mouse skeletal muscle tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse heart tissue, mouse skeletal muscle tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11017-1-AP targets Dystroglycan in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Dystroglycan fusion protein Ag1456|
|Full Name||dystroglycan 1 (dystrophin-associated glycoprotein 1)|
|Calculated molecular weight||97 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC012740|
|Gene ID (NCBI)||1605|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).
|Product Specific Protocols|
|WB protocol for Dystroglycan antibody 11017-1-AP||Download protocol|
|IHC protocol for Dystroglycan antibody 11017-1-AP||Download protocol|
|IF protocol for Dystroglycan antibody 11017-1-AP||Download protocol|
|IP protocol for Dystroglycan antibody 11017-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Ryan (Verified Customer) (01-10-2018)