DARS Polyclonal antibody, PBS Only

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  WB analysis using 14989-1-AP (same clone as 14989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14989-1-AP (DARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14989-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14989-1-AP (same clone as 14989-1-PBS)

  IP result of anti-DARS (IP:14989-1-AP, 4ug; Detection:14989-1-AP 1:5000) with HeLa cells lysate 2160 ug. This data was developed using the same antibody clone with 14989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 14989-1-AP (same clone as 14989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14989-1-AP (DARS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 14989-1-AP (same clone as 14989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14989-1-AP (DARS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14989-1-PBS in a different storage buffer formulation.

DARS Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

14989-1-PBS targets DARS in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6974

  Product name: Recombinant human DARS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 155-501 aa of BC000629

  Sequence: QLDDAVRPEAEGEEEGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP

• Full Name: aspartyl-tRNA synthetase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC000629
• Gene Symbol: DARS
• Gene ID (NCBI): 1615
• RRID: AB_2261349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14868
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.