DARS Polyclonal antibody

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  WB analysis using 14989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14989-1-AP (DARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14989-1-AP

  IP result of anti-DARS (IP:14989-1-AP, 4ug; Detection:14989-1-AP 1:5000) with HeLa cells lysate 2160 ug.

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  IHC staining of rat brain using 14989-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14989-1-AP (DARS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14989-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14989-1-AP (DARS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DARS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14989-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

14989-1-AP targets DARS in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DARS fusion protein Ag6974
• Full Name: aspartyl-tRNA synthetase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC000629
• Gene Symbol: DARS
• Gene ID (NCBI): 1615
• RRID: AB_2261349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14868
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
human,mouse	CoIP	J Virol; Multiple Host Factors Interact with Hypervariable Domain of Chikungunya Virus nsP3 and Determine Viral Replication in Cell-Specific Mode.; Authors - Chetan D Meshram
human	WB	Am J Cancer Res; hsa_circ_0000519 promotes the progression of lung adenocarcinoma through the hsa-miR-1296-5p/DARS axis; Authors - Guangxu Tu