DARS Polyclonal antibody

DARS Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14989-1-AP

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

CoIPSee 1 publications below

Product Information

14989-1-AP targets DARS in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DARS fusion protein Ag6974
Full Name aspartyl-tRNA synthetase
Calculated molecular weight 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberBC000629
Gene symbol DARS
Gene ID (NCBI) 1615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DARS antibody 14989-1-APDownload protocol
IP protocol for DARS antibody 14989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseCoIP

J Virol

Multiple Host Factors Interact with Hypervariable Domain of Chikungunya Virus nsP3 and Determine Viral Replication in Cell-Specific Mode.

Authors - Chetan D Meshram