DAXX Monoclonal antibody

DAXX Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F3G9

Cat no : 67879-1-Ig

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx



Tested Applications

Positive WB detected inRaji cells, HeLa cells, Daudi cells, MOLT-4 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67879-1-Ig targets DAXX in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DAXX fusion protein Ag14606
Full Name death-domain associated protein
Calculated molecular weight 740 aa, 81 kDa
Observed molecular weight100-130 kDa
GenBank accession numberBC109074
Gene symbol DAXX
Gene ID (NCBI) 1616
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

Protocols

Product Specific Protocols
WB protocol for DAXX antibody 67879-1-IgDownload protocol
IHC protocol for DAXX antibody 67879-1-IgDownload protocol
IF protocol for DAXX antibody 67879-1-IgDownload protocol
Standard Protocols
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