DCBLD2 Polyclonal antibody, PBS Only

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  WB analysis using 30245-1-AP (same clone as 30245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30245-1-AP (DCBLD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30245-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30245-1-AP (same clone as 30245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30245-1-AP (DCBLD2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 30245-1-AP (same clone as 30245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30245-1-AP (DCBLD2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30245-1-PBS in a different storage buffer formulation.

DCBLD2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 30245-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

30245-1-PBS targets DCBLD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCBLD2 fusion protein Ag32823
• Full Name: discoidin, CUB and LCCL domain containing 2
• Calculated Molecular Weight: 775 aa, 89 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC029658
• Gene Symbol: DCBLD2
• Gene ID (NCBI): 131566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PD2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).