DCLK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DCLK1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 21699-1-AP

Synonyms

EC:2.7.11.1, Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A

---

Validation Data Gallery

• 

  WB analysis of rat brain using 21699-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21699-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21699-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21699-1-AP (DCLK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 21699-1-AP

  IP result of anti-DCLK1 (IP:21699-1-AP, 4ug; Detection:21699-1-AP 1:1000) with rat brain tissue lysate 4000ug.

• 

  IF Staining of mouse brain using 21699-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (21699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: rat brain tissue, human brain tissue, mouse brain tissue
• Positive IP detected in: rat brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

21699-1-AP targets DCLK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCLK1 fusion protein Ag16292
• Full Name: doublecortin-like kinase 1
• Calculated Molecular Weight: 729 aa, 81 kDa
• Observed Molecular Weight: 48 kDa, 82 kDa
• GenBank Accession Number: BC152456
• Gene Symbol: DCLK1
• Gene ID (NCBI): 9201
• RRID: AB_10859251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

Publications

Species	Application	Title
mouse	IF,FC	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
mouse	IF	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human	WB,IHC,IF,IP	Clin Transl Med; Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.; Authors - Lanqing Wang ; KD Validated
human	WB	J Gastroenterol; High-throughput screening identified miR-7-2-3p and miR-29c-3p as metastasis suppressors in gallbladder carcinoma.; Authors - Kai Lu ; KD Validated
human	WB	Front Pharmacol; XMD-17-51 Inhibits DCLK1 Kinase and Prevents Lung Cancer Progression.; Authors - Wei-Qiang Yang ; KD Validated
human	WB	Cell Physiol Biochem; MicroRNA-195 Suppresses the Progression of Pancreatic Cancer by Targeting DCLK1.; Authors - Bin Zhou