DDAH1 Polyclonal antibody

DDAH1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30108-1-AP

Synonyms

DDAH, DDAH 1, DDAH1, DDAHI, Dimethylargininase 1, FLJ21264, FLJ25539

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Validation Data Gallery

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  WB analysis using 30108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30108-1-AP (DDAH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 30108-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using DDAH1 antibody (30108-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells, SH-SY5Y cells, mouse brain tissue
• Positive IF detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30108-1-AP targets DDAH1 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDAH1 fusion protein Ag32516
• Full Name: dimethylarginine dimethylaminohydrolase 1
• Calculated Molecular Weight: 31kd
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_012137
• Gene Symbol: DDAH1
• Gene ID (NCBI): 23576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.