DDRGK1 Polyclonal antibody, PBS Only

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  WB analysis using 21445-1-AP (same clone as 21445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21445-1-AP (same clone as 21445-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (21445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DDRGK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21445-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

21445-1-PBS targets DDRGK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13742

  Product name: Recombinant human DDRGK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-314 aa of BC000643

  Sequence: MVAPVWYLVAAALLVGFILFLTRSRGRAASAGQEPLHNEELAGAGRVAQPGPLEPEEPRAGGRPRRRRDLGSRLQAQRRAQRVAWAEADENEEEAVILAQEEEGVEKPAETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQAQREHEEYLKLKEAFVVEEEGVGETMTEEQSQSFLTEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTITGVIDDRGKFIYITPEELAAVANFIRQRGRVSIAELAQASNSLIAWGRESPAQAPA

• Full Name: DDRGK domain containing 1
• Calculated Molecular Weight: 314 aa, 36 kDa
• Observed Molecular Weight: 36-46 kDa
• GenBank Accession Number: BC000643
• Gene Symbol: DDRGK1
• Gene ID (NCBI): 65992
• RRID: AB_2827383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HY6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.