DDRGK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDRGK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 21445-1-AP

Synonyms

C20orf116, DDRGK domain containing 1, DDRGK1, UFBP1

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Validation Data Gallery

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  WB analysis using 21445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 21445-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21445-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (21445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-3 cells, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue
• Positive IHC detected in: mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

21445-1-AP targets DDRGK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDRGK1 fusion protein Ag13742
• Full Name: DDRGK domain containing 1
• Calculated Molecular Weight: 314 aa, 36 kDa
• Observed Molecular Weight: 36-46 kDa
• GenBank Accession Number: BC000643
• Gene Symbol: DDRGK1
• Gene ID (NCBI): 65992
• RRID: AB_2827383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

Publications

Species	Application	Title
human	WB	Cell; A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.; Authors - Jin Rui Liang ; KD Validated
human	IHC	Nat Cell Biol; UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination.; Authors - Jiang Liu
		Cell Res; UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis.; Authors - Lihui Wang
human	WB,IHC,IF	Adv Sci (Weinh); DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination; Authors - Xin Wang ; KO Validated
human	WB	Sci Adv; Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control; Authors - Ryosuke Ishimura ; KO Validated
human	WB	Nat Commun; A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1α stability.; Authors - Jiang Liu ; KD Validated