DDX54 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDX54 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26894-1-AP

Synonyms

DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97

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Validation Data Gallery

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  WB analysis of HeLa using 26894-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26894-1-AP (DDX54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 26894-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (26894-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

26894-1-AP targets DDX54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX54 fusion protein Ag25289
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
• Calculated Molecular Weight: 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC156669
• Gene Symbol: DDX54
• Gene ID (NCBI): 79039
• RRID: AB_2880671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

Publications

Species	Application	Title
human	WB,IHC	Am J Cancer Res; LINC00908 attenuates LUAD tumorigenesis through DEAD-box helicase 54; Authors - Jiahua Zhao
human	WB	bioRxiv; Nucleolar dynamics are determined by the ordered assembly of the ribosome; Authors - Jessica Sheu-Gruttadauria ; KD Validated