DEFA5 Polyclonal antibody

DEFA5 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 18268-1-AP

Synonyms

DEF5, DEFA5, Defensin 5, Defensin, alpha 5, defensin, alpha 5, Paneth cell, HD 5

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Validation Data Gallery

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  IHC staining of human small intestine using 18268-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18268-1-AP (DEFA5 antibody) at dilution of 1:3200 (under 10x lens)..

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  IHC staining of human small intestine using 18268-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18268-1-AP (DEFA5 antibody) at dilution of 1:3200 (under 40x lens)..

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Tested Applications

• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18268-1-AP is DEFA5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEFA5 fusion protein Ag12793
• Full Name: defensin, alpha 5, Paneth cell-specific
• Calculated Molecular Weight: 94 aa, 10 kDa
• GenBank Accession Number: BC069690
• Gene Symbol: DEFA5
• Gene ID (NCBI): 1670
• RRID: AB_2878526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEFA5, also named as DEF5, belongs to the alpha-defensin family. It has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Mature DEFA5 is about 5kd. It has a homodimer form.