CoraLite® Plus 488-conjugated DFFA,DFF45 Recombinant monoclonal antibody

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  IF Staining of HEK-293 using CL488-85220-5

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 DFFA,DFF45 antibody (CL488-85220-5, Clone: 242915D6 ) at dilution of 1:200, CL594-Phalloidin (red).

DFFA,DFF45 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85220-5

Clone No.242915D6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

Synonyms

DFF45, DFFA, 242915D6, DFF alpha, DFF1

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85220-5 targets DFFA,DFF45 in applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0244

  Product name: Recombinant human DFFA,DFF45 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 9-328 aa of BC000037

  Sequence: GVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQ

• Full Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC000037
• Gene Symbol: DFFA
• Gene ID (NCBI): 1676
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00273
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.