CoraLite® Plus 488-conjugated DFFA,DFF45 Recombinant antibody

DFFA,DFF45 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-85220-5
Clone No.242915D6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

DFF45, DFFA, 242915D6, DFF alpha, DFF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85220-5 targets DFFA,DFF45 in applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DFFA,DFF45 fusion protein Ag0244 Predict reactive species
Full Name DNA fragmentation factor, 45kDa, alpha polypeptide
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC000037
Gene Symbol DFFA
Gene ID (NCBI) 1676
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00273
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DFFA,DFF45 antibody CL488-85220-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...