Validation Data Gallery
|Positive WB detected in
|L02 cells, HeLa cells, human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue, rat brain tissue, mouse liver tissue, rat heart tissue
|Positive IP detected in
|mouse liver tissue
|Positive IHC detected in
|human liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16431-1-AP targets DLD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|DLD fusion protein Ag9729
|Calculated molecular weight
|509 aa, 54 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
|Product Specific Protocols
|WB protocol for DLD antibody 16431-1-AP
|IHC protocol for DLD antibody 16431-1-AP
|IF protocol for DLD antibody 16431-1-AP
|IP protocol for DLD antibody 16431-1-AP
|Click here to view our Standard Protocols
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OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.
Cell Mol Life Sci
Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (07-12-2023)
Great antibody for WB. Very strong signal and very clean background.