• Featured Product
  • KD/KO Validated

DLD Polyclonal antibody

DLD Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16431-1-AP

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3



Tested Applications

Positive WB detected inL02 cells, HeLa cells, human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue, rat brain tissue, mouse liver tissue, rat heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16431-1-AP targets DLD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLD fusion protein Ag9729
Full Name dihydrolipoamide dehydrogenase
Calculated molecular weight 509 aa, 54 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC018696
Gene symbol DLD
Gene ID (NCBI) 1738
RRIDAB_2091888
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for DLD antibody 16431-1-APDownload protocol
IHC protocol for DLD antibody 16431-1-APDownload protocol
IF protocol for DLD antibody 16431-1-APDownload protocol
IP protocol for DLD antibody 16431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Science

Copper induces cell death by targeting lipoylated TCA cycle proteins.

Authors - Peter Tsvetkov
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
  • KO Validated
humanWB

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
humanWB

Nat Commun

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Authors - Jiliang Xia
  • KD Validated
humanWB

Redox Biol

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.

Authors - Yingying Chen
mouseWB

Cell Mol Life Sci

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Authors - Min-Zhi Peng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and very clean background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Mouse liver
DLD Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:16431-1-AP)