DLD Polyclonal antibody

• Featured Product
• KD/KO Validated

DLD Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 16431-1-AP

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

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Validation Data Gallery

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  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16431-1-AP

  WB result of DLD antibody (16431-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.

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  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16431-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16431-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16431-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16431-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16431-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16431-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16431-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16431-1-AP

  IP Result of anti-DLD (IP:16431-1-AP, 4ug; Detection:16431-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16431-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16431-1-AP (DLD antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, HeLa cells, human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue, rat brain tissue, mouse liver tissue, rat heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

16431-1-AP targets DLD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLD fusion protein Ag9729
• Full Name: dihydrolipoamide dehydrogenase
• Calculated molecular weight: 509 aa, 54 kDa
• Observed molecular weight: 56 kDa
• GenBank accession number: BC018696
• Gene symbol: DLD
• Gene ID (NCBI): 1738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Publications

Species	Application	Title
		Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors - ; KO Validated
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia ; KD Validated
human	WB	Redox Biol; Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.; Authors - Yingying Chen
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng