DMPK Polyclonal antibody

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  WB analysis using 16711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16711-1-AP (DMPK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 16711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 16711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 16711-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DMPK antibody (16711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DMPK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16711-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DMK, DM1PK, DM1 protein kinase, DM1, DM kinase

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Tested Applications

• Positive WB detected in: K-562 cells, SH-SY5Y cells, C2C12 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16711-1-AP targets DMPK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMPK fusion protein Ag10127
• Full Name: dystrophia myotonica-protein kinase
• Calculated Molecular Weight: 629 aa, 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC062553
• Gene Symbol: DMPK
• Gene ID (NCBI): 1760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09013
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMPK (Myotonic Dystrophy Protein Kinase) is a serine-threonine protein kinase, strongly linked to myotonic dystrophy type 1 (DM1), the most prevalent muscular dystrophy in adults (PMID: 19309729). It is best known for its association with myotonic dystrophy type 1 (DM1), a multisystemic genetic disorder characterized by myotonia, muscle wasting, cardiac conduction defects, and other systemic manifestations (PMID: 24136222).