DNAJB13 Polyclonal antibody

DNAJB13 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25118-1-AP

Synonyms

DNAJB13, TSARG3, TSARG5, TSARG6

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Validation Data Gallery

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  WB analysis using 25118-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25118-1-AP (DNAJB13 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 25118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25118-1-AP (DNAJB13 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse testis using 25118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25118-1-AP (DNAJB13 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25118-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25118-1-AP (DNAJB13 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25118-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25118-1-AP (DNAJB13 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 25118-1-AP

  Immunofluorescent analysis of MDCK cells using 25118-1-AP (DNAJB13 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis
• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

25118-1-AP targets DNAJB13 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB13 fusion protein Ag18934
• Full Name: DnaJ (Hsp40) related, subfamily B, member 13
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC153176
• Gene Symbol: DNAJB13
• Gene ID (NCBI): 374407
• RRID: AB_2879906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF	J Assist Reprod Genet; A novel homozygous mutation in DNAJB13-a gene associated with the sperm axoneme-leads to teratozoospermia.; Authors - Mohan Liu
mouse	WB	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	Reprod Biol Endocrinol; DNAJB7 is dispensable for male fertility in mice; Authors - Shun Bai