DNM1 Monoclonal antibody, PBS Only

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  WB analysis of pig brain using 68009-1-Ig (same clone as 68009-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68009-1-Ig (DNM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68009-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68009-1-Ig (same clone as 68009-1-PBS)

  WB result of DNM1 antibody (68009-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNM1 transfected A549 cells. This data was developed using the same antibody clone with 68009-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68009-1-Ig (same clone as 68009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68009-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68009-1-Ig (same clone as 68009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68009-1-Ig (DNM1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68009-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 68009-1-Ig (same clone as 68009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using DNM1 antibody (68009-1-Ig, Clone: 1B2A11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68009-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DNM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68009-1-PBS

Clone No.1B2A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

dynamin 1, 1B2A11, DNM 1, Dynamin I, Dynamin1

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Product Information

68009-1-PBS targets DNM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12932

  Product name: Recombinant human DNM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 502-851 aa of BC063850

  Sequence: RSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPRITISDP

• Full Name: dynamin 1
• Calculated Molecular Weight: 851 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC063850
• Gene Symbol: DNM1
• Gene ID (NCBI): 1759
• RRID: AB_2918756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q05193
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.