DPYD Polyclonal antibody

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  WB analysis of mouse liver using 25690-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25690-1-AP (DPYD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

DPYD Polyclonal Antibody for WB, ELISA

Cat No. 25690-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25690-1-AP targets DPYD in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20885

  Product name: Recombinant human DPYD protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-173 aa of BC008379

  Sequence: MAPVLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFITSIANKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGPINIGGLQQFATETLILAFSLMNHL

• Full Name: dihydropyrimidine dehydrogenase
• Calculated Molecular Weight: 1025 aa, 111 kDa
• Observed Molecular Weight: 111 kDa
• GenBank Accession Number: BC008379
• Gene Symbol: DPYD
• Gene ID (NCBI): 1806
• RRID: AB_3085814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12882
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy.