DRD4 Polyclonal antibody

DRD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28094-1-AP

Synonyms

D(2C) dopamine receptor, D(4) dopamine receptor, D4DR, Dopamine D4 receptor, dopamine receptor D4, DRD4

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Validation Data Gallery

• 

  WB analysis of mouse brain using 28094-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28094-1-AP (DRD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 28094-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 28094-1-AP (DRD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, pig brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

28094-1-AP targets DRD4 in WB, IF, ELISA applications and shows reactivity with Human, Pig, mouse samples.

• Tested Reactivity: Human, Pig, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRD4 fusion protein Ag26930
• Full Name: dopamine receptor D4
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_000797
• Gene Symbol: DRD4
• Gene ID (NCBI): 1815
• RRID: AB_2881058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The family of dopamine receptors (DRs) includes five G protein-coupled receptors (GPCRs)-DRD1, DRD2, DRD3, DRD4 and DRD5-with different anatomical distribution, expression levels, dopamine affinity, signal transduction, and effector targets (PMID:36523297). DRD4 (dopamine receptor D4) influences the postsynaptic action of dopamine and is implicated in many neurological processes, exhibits polymorphism and is one of the most studied genes in connection with psychiatric disorders (PMID:21873960). Moreover, DRD4 modulate glucose metabolism, and protect neurocytes from anoxia/reoxygenation (A/R) injury. Thus, DRD4 might regulate myocardial I/R injury in association with GLUT4-mediated glucose metabolism (PMID:33584304).

Publications

Species	Application	Title
rat	IF	Aging (Albany NY); Sepsis induced cognitive impairments by disrupting hippocampal parvalbumin interneuron-mediated inhibitory network via a D4-receptor mechanism.; Authors - Muhuo Ji