DUPD1 / DUSP27 Polyclonal antibody

DUPD1 / DUSP27 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25259-1-AP

Synonyms

DUPD1, DUSP27, FMDSP

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Validation Data Gallery

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  WB analysis using 25259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25259-1-AP (DUPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25259-1-AP targets DUPD1 / DUSP27 in WB, ELISA applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUPD1 / DUSP27 fusion protein Ag19658
• Full Name: dual specificity phosphatase and pro isomerase domain containing 1
• Calculated Molecular Weight: 220 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC137322
• Gene Symbol: DUPD1
• Gene ID (NCBI): 338599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dupd1also named as Dusp27, has been categorized as an atypical dual-specificity phosphatase (Dusp) capable of dephosphorylating both tyrosine and serine/threonine residues. It's involved in the modulation of intracellular signaling cascades. In skeletal muscle Dupd1 regulates systemic glucose homeostasis by activating AMPK, which is an energy sensor protein kinase. Dupd1 also affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy.