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  • KD/KO Validated

DYNLT1 Polyclonal antibody

DYNLT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11954-1-AP

Synonyms

CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1



Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue, Sp2/0 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11954-1-AP targets DYNLT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNLT1 fusion protein Ag2557
Full Name dynein, light chain, Tctex-type 1
Calculated molecular weight 113 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC029412
Gene symbol DYNLT1
Gene ID (NCBI) 6993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (Pubmed: 19448628)

Protocols

Product Specific Protocols
WB protocol for DYNLT1 antibody 11954-1-APDownload protocol
IHC protocol for DYNLT1 antibody 11954-1-APDownload protocol
IF protocol for DYNLT1 antibody 11954-1-APDownload protocol
IP protocol for DYNLT1 antibody 11954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Nat Neurosci

Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells.

Authors - Gauthier-Fisher Andrée A
  • KD Validated
mouseWB

Cell Res

Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.

Authors - Kong Shanshan S
humanWB

J Cell Biol

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Authors - Hironori Inaba
  • KD Validated
humanIHC

Cancers (Basel)

Dynein Light Chain Protein Tctex1: A Novel Prognostic Marker and Molecular Mediator in Glioblastoma.

Authors - Claudia Alexandra Dumitru
WB

Biochem Pharmacol

Cannabinoid receptor-2 expression modulates Gβ1γ2 protein interaction with the activator of G protein signalling 2/dynein light chain protein Tctex-1.

Authors - Marina Nagler
humanWB,IF

J Cell Physiol

Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis.

Authors - Ola Z Ismail
  • KD Validated