Di-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86890-2-RR (same clone as 86890-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86890-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86890-2-RR (same clone as 86890-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86890-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 86890-2-RR (same clone as 86890-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H3 (Lys27) antibody (86890-2-RR, Clone: 251846E11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86890-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of / using 86890-2-RR (same clone as 86890-2-PBS)

  Dot blot analysis was used to confirm the specificity of 86890-2-RR Di-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 86890-2-PBS in a different storage buffer formulation.

Di-Methyl-Histone H3 (Lys27) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Cat No. 86890-2-PBS

Clone No.251846E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Synonyms

DiMethylHistone H3, Di-Methyl-Histone H3, Di-MethylHistone H3 (Lys27), Di-MethylHistone H3 Lys27, Di-Methyl-Histone-H3

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Product Information

86890-2-PBS targets Di-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.