Di-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody

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  WB analysis using 86890-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86890-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86890-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H3 (Lys27) antibody (86890-2-RR, Clone: 251846E11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of / using 86890-2-RR

  Dot blot analysis was used to confirm the specificity of 86890-2-RR Di-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

Di-Methyl-Histone H3 (Lys27) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Cat No. 86890-2-RR

Clone No.251846E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, ELISA

Synonyms

DiMethylHistone H3, Di-Methyl-Histone H3, Di-MethylHistone H3 (Lys27), Di-MethylHistone H3 Lys27, Di-Methyl-Histone-H3

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293T cells, K-562 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: / /

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86890-2-RR targets Di-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.