Various lysates were subjected to SDS PAGE followed by western blot with 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse colon using 87006-1-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 87006-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H4 (Lys20) antibody (87006-1-RR, Clone: 251934G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H4 (Lys20) antibody (87006-1-RR, Clone: 251934G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Dot Blot experiment of / using 87006-1-RR
Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H4 (Lys20) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H4 (Lys20) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
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mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Positive Dot Blot detected in
/ /
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
DOT BLOT
DOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
87006-1-RR targets Di-Methyl-Histone H4 (Lys20) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
Various lysates were subjected to SDS PAGE followed by western blot with 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse colon using 87006-1-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 87006-1-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87006-1-RR (Di-Methyl-Histone H4 (Lys20) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 87006-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H4 (Lys20) antibody (87006-1-RR, Clone: 251934G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
DOT BLOT Figures
Dot Blot experiment of / using 87006-1-RR
Dot blot analysis was used to confirm the specificity of 86990-2-RR Di-Methyl-Histone H4 (Lys20) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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