Digoxin Recombinant monoclonal antibody

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  ELISA experiment of Digoxin using 86100-7-RR

  ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 100 µl per well of Digoxin-BSA, diluted in carbonate/bicarbonate buffer, at a concentration of 0.7 µg/mL (2hr,37℃). The plate was washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of Digoxin Recombinant Monoclonal Antibody (Product # 86100-7-RR) and serially diluting it with a starting concentration of 1 µg/mL (1 hr,37℃). The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:10,000 (1 hr, 37℃). Detection was performed using an Ultra TMB Substrate (3-5 min, room temp). The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Digoxin Uni-rAb^® Recombinant Antibody for ELISA

Cat No. 86100-7-RR

Clone No.250496G6

Host / Isotype

Rabbit / IgG

Reactivity

-

Applications

ELISA

Synonyms

20830-75-5, CasNo 20830-75-5, 地高辛

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Tested Applications

• Positive ELISA detected in: Digoxin

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 0

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86100-7-RR targets Digoxin in ELISA applications and shows reactivity with - samples.

• Tested Reactivity: -
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Digoxin
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is designed for Digoxin. Digoxin is a cardiac glycoside extracted from the leaves of digitalis (Digitalis lanata). Its mechanism of action mainly involves enhancing myocardial contractility, slowing heart rate, and reducing sympathetic nerve activity.