DjC8 Polyclonal antibody

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  WB analysis using 32709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32709-1-AP (DNAJC8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

DjC8 Polyclonal Antibody for WB, ELISA

Cat No. 32709-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DnaJ homolog subfamily C member 8, DNAJC8, HSPC315, HSPC331, SPF31

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Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32709-1-AP targets DjC8 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38520

  Product name: Recombinant human DNAJC8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-253 aa of NM_014280.2

  Sequence: MAASGESGTSGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFEVLQIDPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQAGKEYVEHTVKERKKQLKKEGKPTIVEEDDPELFKQAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGRVDSWRNFQANTKGKKEKKNRTFLRPPKVKMEQRE

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 8
• Calculated Molecular Weight: 30kDa，253aa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_014280.2
• Gene Symbol: DNAJC8
• Gene ID (NCBI): 22826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O75937
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DjC8 (C8, a member of DNAJ heat shock protein family), also known as DNAJC8, which is a highly conserved protein, belonging to heat shock protein 40(HSP40) family. The protein is expressed in many cell types, including central nervous system macrophages, cerebellar inhibitory neurons, choroid plexus epithelial cells of ventricles, oligodendrocyte precursor cells and so on. In normal tissues, the expression level of DjC8 is relatively low, but it is high in some tissues such as testis, skeletal muscle and spleen. In hepatocellular carcinoma, the high expression of DjC8 is related to poor prognosis. Knocking down DjC8 can inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells, and reduce the IC50 value of sorafenib, indicating that it may be a potential therapeutic target and prognostic marker for hepatocellular carcinoma. In addition, DjC8 is regulated by TIG1 in cervical cancer and participates in glycolysis. DjC8 contains a conserved J domain, which can interact with HSP70 and activate its ATPase activity, thus participating in the folding, unfolding, transport and degradation of protein. The molecular weight of DjC8 is 30 kDa.