Anti-Mouse CD324 (E-cadherin) (DECMA-1)

CD324 (E-cadherin) Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse, Human, Canine

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

DECMA-1

Cat no : 65241-1-Ig

Synonyms

AA960649, cadherin 1, Cdh1, Ecad, L CAM, Um, UVO



Tested Applications

Positive IF detected inmouse colon tissue
Positive FC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:625-1:2500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65241-1-Ig targets CD324 (E-cadherin) in IF, FC applications and shows reactivity with Mouse, Human, Canine samples.

Tested Reactivity Mouse, Human, Canine
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse embryonal carcinoma cell line PCC4 Aza RI
Full Name cadherin 1
GenBank Accession NumberBC098501
Gene Symbol Cdh1
Gene ID (NCBI) 12550
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
IF protocol for CD324 (E-cadherin) antibody 65241-1-IgDownload protocol
FC protocol for CD324 (E-cadherin) antibody 65241-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols