EBP50/NHERF-1 Recombinant monoclonal antibody

• 

  WB analysis using 87174-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87174-1-RR (SLC9A3R1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 87174-1-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 87174-1-RR (SLC9A3R1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87174-1-RR against Human EBP50/NHERF-1 were performed. The affinity constant is 2.85 nM.

EBP50/NHERF-1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87174-1-RR

Clone No.252465A2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

SLC9A3R1, EBP50, NHERF-1

---

Tested Applications

• Positive WB detected in: HCT 116 cells, rat kidney tissue, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87174-1-RR targets EBP50/NHERF-1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30878

  Product name: Recombinant human SLC9A3R1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 260-358 aa of NM_004252

  Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_004252
• Gene Symbol: SLC9A3R1/EBP50/NHERF-1
• Gene ID (NCBI): 9368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14745
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).