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ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11305-1-AP

Synonyms

ECHS1, ECoH-1, Enoyl CoA hydratase 1, SCEH



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HT-1080 cells, human liver tissue, L02 cells, mouse liver tissue, PC-3 cells, rat heart tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:25-1:200
Sample-dependent, check data in validation data gallery

Product Information

11305-1-AP targets ECHS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECHS1 fusion protein Ag1842
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated molecular weight 31 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC008906
Gene symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Protocols

Product Specific Protocols
WB protocol for ECHS1 antibody 11305-1-APDownload protocol
IHC protocol for ECHS1 antibody 11305-1-APDownload protocol
IF protocol for ECHS1 antibody 11305-1-APDownload protocol
IP protocol for ECHS1 antibody 11305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Med Rep

Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo.

Authors - Bi-Yun Lin
humanWB

Nephron Exp Nephrol

Uric Acid-induced endothelial dysfunction is associated with mitochondrial alterations and decreased intracellular ATP concentrations.

Authors - Sánchez-Lozada Laura Gabriela LG
humanWB,IF

Arch Virol

Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.

Authors - Gong Xianqiong X
mouseWB

Lipids Health Dis

Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice.

Authors - Ching-Chou Tsai
ratWB

Metabolism

Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake.

Authors - Roncal-Jimenez Carlos A CA
mouseWB

Am J Physiol Regul Integr Comp Physiol

The Day/Night Proteome in the Murine Heart.

Authors - Peter Podobed