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  • KD/KO Validated

ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11305-1-AP


ECHS1, ECoH-1, Enoyl CoA hydratase 1, SCEH

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse heart, mouse liver, rat liver
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11305-1-AP targets ECHS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECHS1 fusion protein Ag1842
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)


Product Specific Protocols
WB protocol for ECHS1 antibody 11305-1-APDownload protocol
IHC protocol for ECHS1 antibody 11305-1-APDownload protocol
IF protocol for ECHS1 antibody 11305-1-APDownload protocol
IP protocol for ECHS1 antibody 11305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Res

One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis.

Authors - Yeung Anthony T AT

J Proteome Res

Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.

Authors - Cieniewski-Bernard Caroline C

J Proteome Res

Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.

Authors - Gek San Tan


Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang

Am J Pathol

The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.

Authors - Addabbo Francesco F

J Med Genet

Strategic validation of variants of uncertain significance in ECHS1 genetic testing

Authors - Yoshihito Kishita
  • KO Validated