|Positive WB detected in||HepG2 cells, HeLa cells, HT-1080 cells, human liver tissue, L02 cells, mouse liver tissue, PC-3 cells, rat heart tissue|
|Positive IP detected in||PC-3 cells|
|Positive IHC detected in||human prostate cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:25-1:200|
|Sample-dependent, check data in validation data gallery|
11305-1-AP targets ECHS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ECHS1 fusion protein Ag1842|
|Full Name||enoyl Coenzyme A hydratase, short chain, 1, mitochondrial|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||29-31 kDa|
|GenBank accession number||BC008906|
|Gene ID (NCBI)||1892|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
|Product Specific Protocols|
|WB protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|IHC protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|IF protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|IP protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Med Rep
Enoyl-coenzyme A hydratase short chain 1 silencing attenuates the proliferation of hepatocellular carcinoma by inhibiting epidermal growth factor signaling in vitro and in vivo.
Nephron Exp Nephrol
Uric Acid-induced endothelial dysfunction is associated with mitochondrial alterations and decreased intracellular ATP concentrations.
Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.
Lipids Health Dis
Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice.
Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake.
Am J Physiol Regul Integr Comp Physiol
The Day/Night Proteome in the Murine Heart.