Validation Data Gallery
|Positive WB detected in||HepG2 cells, HeLa cells, mouse heart, mouse liver, rat liver|
|Positive IP detected in||PC-3 cells|
|Positive IHC detected in||human prostate cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11305-1-AP targets ECHS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ECHS1 fusion protein Ag1842|
|Full Name||enoyl Coenzyme A hydratase, short chain, 1, mitochondrial|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||29-31 kDa|
|GenBank accession number||BC008906|
|Gene ID (NCBI)||1892|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
|Product Specific Protocols|
|WB protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|IHC protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|IF protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|IP protocol for ECHS1 antibody 11305-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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