Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, rat liver tissue, L02 cells, rat heart tiisue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human colon tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11305-1-AP targets ECHS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1842 Product name: Recombinant human ECHS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC008906 Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ Predict reactive species |
| Full Name | enoyl Coenzyme A hydratase, short chain, 1, mitochondrial |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 29-31 kDa |
| GenBank Accession Number | BC008906 |
| Gene Symbol | ECHS1 |
| Gene ID (NCBI) | 1892 |
| RRID | AB_2262166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30084 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| IHC protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| IP protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| WB protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
11305-1-AP is an anti-ECHS1 antibody with 38 total citations. Citations appear in journals including Nature Communications, EMBO Journal, Cancer Research, Drug Resistance Updates, Metabolism, Journal of Biological Chemistry, iScience, Free Radical Biology and Medicine, and PLoS ONE. Research fields span lipid and fatty acid metabolism, mitochondrial function, hepatocellular and colorectal cancer, liver disease (MASLD/NAFLD), cardiovascular biology, neurology, nephrology, and proteomics.
| Species | Application | Title |
|---|---|---|
Cancer Res One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis. | ||
Drug Resist Updat MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance | ||
Nat Commun Hypoxia-induced downregulation of PGK1 crotonylation promotes tumorigenesis by coordinating glycolysis and the TCA cycle | ||
Metabolism Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake. | ||
J Exp Clin Cancer Res Acetylation-induced degradation of ECHS1 enhances BCAA accumulation and proliferation in KRAS-mutant colorectal cancer | ||
EBioMedicine Integrated spatial multi-omics delineates fatty acid degradation fuels malignant evolution at the tumour periphery in cervical squamous cell carcinoma.
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